PCR Clean-Up Kit is designed to purify single- or double-stranded DNA fragments from PCR and other enzymatic reactions. Fragments ranging from 100 bp to 10 kb can be purified from primers, nucleotides, polymerases and salts using mini columns in a microcentrifuge.
PCR Clean-Up Kit is designed for a rapid and efficient purification of DNA fragments after enzymatic reactions. It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, and other contaminants. The purified DNA can be used in common downstream applications.The kit enables the purification of DNA fragments from 50 bp to 20 kb. However purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA.
PROTOCOL EXECUTION TIME
ADDITIONAL REAGENTS AND EQUIPMENT REQUIRED
> 96–100% ethanol PFA
> 3 M sodium acetate pH 5.0
> 1.5–2.0 ml sterile microcentrifuge tubes
> microcentrifuge with rotor for 1.5–2.0 ml (≥ 11,000 x g)
> racks for 1.5 and 2.0 ml microcentrifuge tubes
> utomatic pipettes and pipette tips (in range 2-20, 20-100, and 100-1000 μl)
> disposable gloves