Description

DNA Gel-Out Kit is designed for a rapid and efficient purification of DNA fragments kb from standard or lowmelt agarose gels in TAE or TBE buffer. The DNA purification procedure utilizes spin mini columns with membranes which efficiently and selectively bind nucleic acids. In the first step, the DNA fragment is excised from an agarose gel and incubated in GO-G Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, the binding buffer contains a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The washing stage efficiently removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer (GO-E Buffer), distilled water or TE Buffer. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 50 bp to 20 kb. However purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA.